만성 치주염과 급속 파괴성 치주염에서의 흑색색소 형성 Bacteroides의 분포도 비교
Comparison of Black-pigmented Bacteroides using 16S rRNA analysis between Chronic periodontitis and Aggressive Periodontitis
소속 상세정보
신철우
최봉규/문익상/김종관/조규성
KMID : 0363020040340020255
Abstract
The purpose of the research is to compare the distribution of Black-pigmented Bacteroides between Chronic Periodontitis and Aggressive Periodontitis.
P. gingivalis, P. intermedia and P. nigrescens were examined in order to evaluate their distribution in patients with Chronic Periodontitis(CP) and Aggressive Periodontitis(AP).
PCR and dot-blots hybridization of 16S rRNA gene were used to compare bacterial distribution of two groups - CP group and AP group, which were divided into two subgroups. Subgingival plaque taken from the diseased sites(pocket depth≥6 mm) and healthy sites(pocket depth≤3 mm) were grouped into the experimental group and the control group.
The result are as follows ;
1. The distribution of P. gingivalis was 98.33% for chronic Periodotitis(CP), 94.17% for Aggressive Periodontitis(AP), the distribution of P. intermedia was 77.50% for CP, 64.17% for AP, and the distribution of P. nigrescens was 35.00%, 29.17%.
In all 3 types of bacteria, CP group showed higher distribution compared to AP group, but only P. intermedia showed statistically significant difference.
2. In the case of CP, every type of bacteria showed higher distribution in the experimental group with statistically significant difference.
3. In the case of AP, every type of bacteria also showed higher distribution in the experimental group, but P. gingivalis and P. .intermedia showed the result with statistically significant difference, and the other did not
4. In 3 all bacteria type, N-AP showed higher distribution than N-CP without statistically significant difference
These results suggest that the comparison of the distribution of Bacteroides between Chronic Periodontitis and Aggressive Periodontitis has no statistically significant difference, except P. intermedia.
The purpose of the research is to compare the distribution of Black-pigmented Bacteroides between Chronic Periodontitis and Aggressive Periodontitis.
P. gingivalis, P. intermedia and P. nigrescens were examined in order to evaluate their distribution in patients with Chronic Periodontitis(CP) and Aggressive Periodontitis(AP).
PCR and dot-blots hybridization of 16S rRNA gene were used to compare bacterial distribution of two groups - CP group and AP group, which were divided into two subgroups. Subgingival plaque taken from the diseased sites(pocket depth≥6 mm) and healthy sites(pocket depth≤3 mm) were grouped into the experimental group and the control group.
The result are as follows ;
1. The distribution of P. gingivalis was 98.33% for chronic Periodotitis(CP), 94.17% for Aggressive Periodontitis(AP), the distribution of P. intermedia was 77.50% for CP, 64.17% for AP, and the distribution of P. nigrescens was 35.00%, 29.17%.
In all 3 types of bacteria, CP group showed higher distribution compared to AP group, but only P. intermedia showed statistically significant difference.
2. In the case of CP, every type of bacteria showed higher distribution in the experimental group with statistically significant difference.
3. In the case of AP, every type of bacteria also showed higher distribution in the experimental group, but P. gingivalis and P. .intermedia showed the result with statistically significant difference, and the other did not
4. In 3 all bacteria type, N-AP showed higher distribution than N-CP without statistically significant difference
These results suggest that the comparison of the distribution of Bacteroides between Chronic Periodontitis and Aggressive Periodontitis has no statistically significant difference, except P. intermedia.
키워드
Black-pigmented Bacteroides;16S rRNA analysis;Chronic periodontitis;Aggressive periodontitis
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